Opening up cryoEM: The Rosalind Franklin Institute commits to fund new detector for lower energy cryoEM
The Rosalind Franklin Institute has committed to developing a multi-million pound technology which enables a more accessible form of electron cryo-microscopy.
The development project is part of a new initiative in collaboration with Nobel prize winner and cryoEM pioneer Dr Richard Henderson and his colleague Dr Chris Russo, both from the MRC Laboratory of Molecular Biology (MRC LMB) in Cambridge.
The goal of the project is to develop a detector which can work at a much lower energy, 100 keV, versus the current industry standard 300 keV, to obtain atomic scale images of biological samples.
Crucially, the new detector will produce better data from the lower energy, 100 keV microscope than existing detectors working with higher energy microscopes.
Professor Richard Henderson, University of Cambridge. Photo credit: MRC-LMB
CryoEM currently requires large and expensive technology, which is housed in highly specialist environments. Lowering the energy of the electrons enables the use of a simpler, and cheaper, electron gun. The new detector, named C100, is the critical first step in this democratisation of the method.
The ultimate aim is to encourage faster scientific progress by bringing a quick, simple and reliable cryoEM to medical research laboratories and drug companies around the world, allowing a massive shift in the accessibility of this revolutionary technique.
An STFC prototype sensor optimised for high speed imaging of electrons at 100keV, supported by the STFC Centre for Instrumentation programme . Photo credit: STFC
In the UK, for example, at the national cryoEM facility, there is a six week wait to get any time on a 300 keV microscope, and a very significant amount of preliminary data is required.
Making the technique accessible to more people increases the opportunities for new advances, says Dr Henderson: “Bringing down the barriers to access these microscopes means we can bring cryoEM to many, many more academics around the world so they can look at samples in more detail, try new approaches and improve our understanding of human diseases.”
The development of this new detector builds upon earlier proof-of-principle work at the MRC LMB. Assisted by LMB’s scientific workshop, Dr Russo’s group built a prototype 100 keV microscope that resolved five structures in seven days, but it was clear that an optimised detector was required to fully realise the potential of the new microscope design.
The Detector and Electronics Group at the Science and Technology Facilities Council have been selected as development partners.
The project is supported as a key component of the Structural Biology theme at the Rosalind Franklin Institute, funded through UK Research and Innovation by the Engineering and Physical Sciences Research Council. The Franklin’s mission is to develop and apply disruptive new technologies in the physical and engineering sciences to transform the UK’s life science research and pharma sector. Creating new drugs has never been slower or more expensive, but the need is growing faster than ever. The use of cryoEM to study biological samples – the development of which won Dr Henderson his Nobel Prize – is a key technology in the process.
Professor James Naismith, Director of the Rosalind Franklin Institute says: “When Richard first told me about their idea for a revolution in accessibility of cryoEM I knew this was exactly the kind of problem that The Rosalind Franklin Institute exists to help solve. C100 is a perfect example of paradigm shifting innovation in life sciences.”
LMB Director Jan Löwe commented, “Making cryo-EM better and more accessible will enable it to be used by everyone to understand biological mechanisms and diseases, and to develop drugs more easily, even when the most complex and currently intractable molecules are involved. Decades of development at LMB and collaboration with STFC have helped create this truly unique opportunity. We are very excited for The Rosalind Franklin Institute to join in to take this work to the next level, given the Franklin’s strong focus on the interface of physics and engineering to enhance biomedical sciences.”
The Rosalind Franklin Institute is a national institute dedicated to transforming life science through interdisciplinary research and technology development. The Institute will bring together researchers in life, physical science, and engineering, to develop disruptive new technologies designed to tackle major challenges in health and life sciences. Focussing initially on five major research themes, the Institute will have significant impact in imaging, diagnostics, drug development, and many more fields.
The Franklin is funded through the UK Research and Innovation through the Engineering and Physical Sciences Research Council (EPSRC). The Institute is an independent organisation founded by the UK Research and Innovation, ten UK universities, and Diamond Light Source, and will have its central hub at the Harwell Campus.
The Rosalind Franklin Institute is a company limited by guarantee registered in England and Wales, registration number 11266143. We are a Registered Charity, number 1179810.
MRC Laboratory of Molecular Biology
The Medical Research Council (MRC) Laboratory of Molecular Biology (LMB) is one of the world’s leading research institutes. Discoveries and inventions developed at the LMB, for example DNA sequencing and methods to determine the structure of proteins, have revolutionised all areas of biology. Its scientists work to advance understanding of biological processes at the molecular level. This information will help us to understand the workings of complex systems, such as the immune system and the brain, and solve key problems in human health.
The Science and Technology Facilities Council is part of UK Research and Innovation – the UK body which works in partnership with universities, research organisations, businesses, charities, and government to create the best possible environment for research and innovation to flourish. STFC funds and supports research in particle and nuclear physics, astronomy, gravitational research and astrophysics, and space science and also operates a network of five national laboratories as well as supporting UK research at a number of international research facilities including CERN, FERMILAB, the ESO telescopes in Chile and many more. Visit stfc.ukri.org for more information.
The Engineering and Physical Sciences Research Council (EPSRC) is the main funding body for engineering and physical sciences research in the UK. By investing in research and postgraduate training, we are building the knowledge and skills base needed to address the scientific and technological challenges facing the nation.
Our portfolio covers a vast range of fields from healthcare technologies to structural engineering, manufacturing to mathematics, advanced materials to chemistry. The research we fund has impact across all sectors. It provides a platform for future UK prosperity by contributing to a healthy, connected, resilient, productive nation.
EPSRC is part of UK Research and Innovation, a new body which works in partnership with universities, research organisations, businesses, charities, and government to create the best possible environment for research and innovation to flourish. We aim to maximise the contribution of each of our component parts, working individually and collectively. We work with our many partners to benefit everyone through knowledge, talent and ideas. For more information visit UK Research and Innovation.