Biophotonic Correlative Optical Platform (BioCOP)

Quantitative correlative imaging of biological processes has now become mission critical in the biomedical sciences. In the recent years, state-of-the-art research has repeatedly demonstrated that the understanding of living systems demands technology with the capabilities to monitor dynamic processes over multiple length- and time-scales; dissecting the functioning of living cells within their tissue microenvironment and the context of human health and disease.

Novel developments such as super-resolution SIM and the LLSM technologies are currently transforming how living single cells and tissue can be minimally invasively studied at unprecedented spatiotemporal resolution, shaping expectations that these will replace current imaging technologies such as confocal and widefield microscopy.

The Franklin is developing the first of its kind Biophotonic Correlative Optical Platform (BioCOP), in collaboration with the Kennedy Institute for Rheumatology at the University of Oxford. This system will allow high performance co-incidence and correlation imaging over multiple lengths and time-scales, featuring a combination of fast high-throughput three-dimensional Lattice Light Sheet Microscopy (LLSM), super-resolution 3D Structural Illumination Microscopy (SIM), and minimally invasive long-term imaging of microfluidics organs on a chip at extended spatiotemporal resolution. These features will allow the study of primary immune cell cultures in the context of human health and disease.

Project team members at The Franklin:

Marco Fritzsche

Angus Kirkland

Project Partners:

The Kennedy Institute for Rheumatology at the University of Oxford