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Protein Production UK

The genomics revolution has opened the protein world for discovery research in medicine, biosciences, and biotechnology. Interpreting and exploiting this information necessitates an understanding of how proteins function as part of complex and dynamic macro-molecular assemblies in cells. Interpreting and exploiting this information necessitates an understanding of how proteins function as part of complex and dynamic macro-molecular assemblies.

Protein Production UK aims to address the production, modification and hence tracking of proteins and their interactions both in vitro and in vivo. This work builds on foundations laid down by PPUK’s predecessor – the Oxford Protein Production Facility – which developed workflows for high throughput cloning, expression, and purification of recombinant proteins that we have implemented in the Franklin.

A key new development is the establishment of a technology platform for the generation of nanobodies for applications in structural cell biology and as potential disease modifying agents.

Laboratory Protocols

Please find below a series of PPUK standard laboratory protocols, we hope you find them useful. If you have any questions please contact info@rfi.ac.uk.

 

Protocol for expression vector construction by In-FusionTM

Protocol for transient expression and purification of secreted proteins from Expi293TM cells

Protocol for baculovirus expression and purification of proteins in insect cells

 

 

Rosalind Franklin Institute